Development Of Short Length Oligonucleotide (DNA & MIRNA) Biomarkers...
GC001A-14SBS: Development Of Short Length Oligonucleotide (DNA & MIRNA) Biomarkers For Meat, Fish Amphibian, Reptiles, Plant and Pathogen Species For Biosensors and Microarray
Abstract:
Authentication of declared components in food is an ever increasing public demand and also a key priority in policy making and regulatory bodies since it is necessary for the safeguard of public health, consumers’ lifestyle, food choice, religious faith, fair-trade economy and wildlife in natural habitats. The turnover of halal food items has crossed USD 661 billion and its expanding rapidly even among the non-Muslim consumers because of its perceived quality attributes and significantly reduced risk to be a carrier of zoonotic diseases. It has been believed that the causative agent of one of the most fatal human disease acquired immune deficiency syndrome was transmitted to human race from African Chimpanzee which is forbidden for consumption in Islam. Perceiving the huge opportunities of the halal food markets, even the European food industries are investing in the production of halal foods. Technologically species-specific PCR seems to be the best and is considered as a robust method in comparison with other methods such as single nucleotide polymorphism (SNP) analysis, PCR-RFLP, PCR-RAPD and DNA barcoding.
Among the PCR assay, multiplex PCR assays are specially interesting since they offer opportunity to detect multiples species is a single assay platform. Some recently published multiplex PCR assay documented for the identification of various animal species.
Research Objectives:
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To develop short-length DNA and mRNA biomarker for multiple haram meat specious such as pork, rat, cat and monkey, dog, frog, reptiles, amphibians, snake, rice, what, vegetables and food-borne microbes (Salmonella, E-coli, H5N1, HIV etc.)
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To test the specificity and efficiency of the developed biomarkers using well-established techniques such as PCR, PCR-RFLP and TaqMan real time PCR.
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To validate the biomarkers for the analysis of process foods.
Methodology:
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Biomarker Development
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Specify and efficiency testing and the designed biomarkers- End-point PCR, Multiplex Real-time PCR and PCR-RFLP.
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Method validation and food analysis
Sub-programme Leader
Assoc. Prof. Dr. Md Eaqub Ali
Co-Researchers:
Dr. Subha Bhassu & Prof. Dr. Sharifah Bee Abd Hamid
Outputs:
ISI-Indexed Journals
SCOPUS